The free radicals are having much importance in the body and which they lead to cell degradation via different path ways. To avoid the free radical interaction with the cells in the body the spin trapping agents normally nitraso compounds are used to trap them later which they leads to formation of Spin adduct easily excreted by the body. The current research concerned with the cell line analysis to investigate cytotoxicity extent determination by MTT assay. The cell lines initially subjected to the MTT assay to determine the cytotoxicity effect of PBN spin trap on the cell lines
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